Document Type
Article
Publication Date
2014
Keywords
Pt(II) complexes, N, S-donor ligand, DNA binding, DNA cleavage, Unwinding, Cytotoxicity
Abstract
The platinum(II) complexes with N, S-donor ligands have been synthesized and characterized by physicochemical methods viz. elemental, electronic, FT-IR, 1H NMR and LC–MS spectra. The binding mode and potency of the complexes with HS DNA (Herring Sperm) have been examined by absorption titration and viscosity measurement studies. The results revealed that complexes bind to HS DNA via covalent mode with the intrinsic binding constant (Kb) in the range 1.37–7.76 × 105 M−1. Decrease in the relative viscosity of HS DNA also supports the covalent mode of binding. The DNA cleavage activity of synthesized complexes has been carried out by gel electrophoresis experiment using supercoiled form of pUC19 DNA; showing the unwinding of the negatively charged supercoiled DNA. Brine shrimp (Artemia Cysts) lethality bioassay technique has been applied for the determination of toxic property of synthesized complexes in terms of μM.
Faculty
Pilon School of Business
Journal
Spectrochim Acta A Mol Biomol Spectrosc
Volume
127
First Page
261
Last Page
267
Version
Publisher's version
Peer Reviewed/Refereed Publication
yes
Terms of Use
Terms of Use for Works posted in SOURCE.
Copyright
© Elsevier B.V.
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
Original Publication Citation
Patel, M. N., Patel, C. R., Joshi, H. N., Thakor, K. P. (2014). DNA interaction and cytotoxic activities of square planar platinum(II) complexes with N, S -donor ligands. Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, 127, 261 - 267. https://doi.org/10.1016/j.saa.2014.02.053
SOURCE Citation
Patel, Mohan N.; Patel, Chintan R.; Joshi, Hardik; and Thakor, Khyati P., "DNA Interaction and Cytotoxic Activities of Square Planar Platinum(II) Complexes with N, S-donor Ligands" (2014). Publications and Scholarship. 28.
https://source.sheridancollege.ca/pilon_publ/28